Mouse anti-Human PARK7 Monoclonal Antibody (Clone K94023_15B1)

ELISA: Microtiter wells were coated with K94023_15B1 at 3 ug/mL as the capture antibody. PARK7 (Cat. PP-363)was used as the antigen. Peroxidase conjugated mouse anti-human PARK7 monoclonal antibody (K92021_20F2) was used as the detection antibody. Result: K94023_15B1 and K92021_20F2 can be used as a matched antibody pair to detect and quantify the concentration of PARK7.

Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug of K94023_15B1 with HeLa lysate containing 200 ug of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. PARK7 (K94023_15B1) at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: HeLa lysate Lane 2: PARK7 immunoprecipitated from HeLa lysate by K94023_15B1 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K94023_15B1 can immunoprecipitate PARK7.

Western Blot: 15 ug of HeLa lysate was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K94023_15B1 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. PARK7 band was visualized using ECL Western Blotting Substrate. Result: K94023_15B1 can detect PARK7 by Western blotting.