Home/ Products / Mouse Monoclonal Abs
WZA2558-100ul
100uL
$540
| target | Crotonyl Lysine |
| host | Mouse |
| clonality | Monoclonal |
| clone number | K16287_14G5 |
| iso type | IgG2a |
| conjugate | Unconjugated |
| applications | IP |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | IP 1:100 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug of K16287_14G5 with crotonic acid treated HeLa lysate containing 200 ug of total protein. After absorption with Protein G beads the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. OC-180 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (light chain specific for membrane above 43 kDa and heavy chain specific for membrane below 43 kDa) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: HeLa lysate treated with crotonic acid (10 mM 16 h) Lane 2: Crotonyl Lysine immunoprecipitated by K16287_14G5 from the same lysate as Lane 1 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K16287_14G5 can immunoprecipitate crotonylated proteins.
WZA2558-100ul
100uL
$540