Home/ Products / Mouse Monoclonal Abs
WZA5417-100ul
100uL
$540
| target | Crotonyl Lysine |
| host | Mouse |
| clonality | Oligoclonal |
| iso type | IgG |
| conjugate | Unconjugated |
| applications | IP |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | IP 1:100 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug of OC-181 with crotonic acid treated HeLa lysate containing 200 ug of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. OC-180 at 1 ug/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: HeLa lysate treated with crotonic acid (10mM, 16h) Lane 2: Crotonyl Lysine immunoprecipitated by OC-181 from the same lysate as Lane 1 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: OC-181 can immunoprecipitate crotonylated protein.
WZA5417-100ul
100uL
$540