Home/ Products / Mouse Monoclonal Abs
WZA5416-100ul
100uL
$480
| target | Crotonyl Lysine |
| host | Mouse |
| reactivity | Human |
| clonality | Oligoclonal |
| iso type | IgG |
| conjugate | Unconjugated |
| applications | WB |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | WB 1:1000 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Western Blot: HeLa cells treated with or without crotonic acid. Lysates were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. OC-180 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Crotonylated protein bands were visualized using ECL Western Blotting Substrate. Lane 1: 15 ug of HeLa lysate Lane 2: 15 ug of crotonic acid (10mM, 16h) treated HeLa lysate Result: OC-180 can detect crotonylated proteins by Western blotting.
WZA5416-100ul
100uL
$480