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WZA4831-100ul
100uL
$865
| target | IL1A |
| host | Mouse |
| gene id | 3552 |
| reactivity | Human |
| clonality | Monoclonal |
| clone number | K24011_18D3 |
| iso type | IgG2b |
| conjugate | Unconjugated |
| applications | IHC, ICC, IP, ELISA |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | IHC 1:200, IP 1:100, ELISA 1:250-1:500 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
ELISA: Microtiter wells were coated with K24011_18D3 at 3 ug/mL as the capture antibody. IL1A (Cat. PP-064) was used as the antigen. Peroxidase conjugated mouse anti-human IL1A monoclonal antibody (K24011_17E10) was used as the detection antibody. Result: K24011_18D3 and K24011_17E10 can be used as a matched antibody pair to detect and quantify the concentration of IL1A.
Immunohistochemistry: IHC-P analysis of liver tissue by IL1A antibody (K24011_18D3). IHC-P was performed using sections of the formalin-fixed paraffin-embedded liver tissue.
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug K24011_18D3 with PMA and LPS treated THP-1 lysate containing 200 ug of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. IL1A (K24011_12H10) at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: PMA and LPS treated THP-1 lysate Lane 2: IL1A immunoprecipitated from PMA and LPS treated THP-1 lysate by K24011_18D3 Lane 3: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K24011_18D3 can immunoprecipitate IL1A.
WZA4831-100ul
100uL
$865