Human SIGLEC7 / Siglec-7 (Gln19-Leu353) protein, with C-Fc(A3) Tag, produced in HEK293

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PRODUCT

  • SKU

    YCD1487-50ug

  • Size

    50ug

  • Price

    $540

1
  • SKU

    YCD1487-500ug

  • Size

    500ug

  • Price

    $4,635

1
This recombinant protein contains Gln19-Leu353 of UniProt Q9Y286. It has a Fc (L118A, G120A, E201A) tag at the C-terminus. The protein's calculated molecular weight is 63.3 kDa, migrating at 70-90 kDa in reducing SDS-PAGE due to glycosylation. Mutations (L118A, G120A, E201A /EU number: L235A/G237A/E318A) in human immunoglobulin G1 (hIgG1) Fc strongly reduce binding of the Fc mutant to cell expressed FcgammaRs, resulting in an almost 4-fold reduction in ADCC compared to that of wild type human IgG1.
gene name SIGLEC7
gene id UniProt Q9Y286
host species Human
production host HEK293
tags C-Fc(A3)
purity > 95% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in 25 mM MES, 150 mM NaCl, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Human Siglec-7 protein

ELISA: Immobilized Human Siglec-7, Fc (L118A, G120A, E201A) Tag at 5 ug/mL (100 uL/well) binds to Neu5Aca2-6GalNAca-PAA-biotin with a linear range of 0.02-0.313 ng/mL.

Related Products

PRODUCT

  • SKU

    YCD1487-50ug

  • Size

    50ug

  • Price

    $540

1
  • SKU

    YCD1487-500ug

  • Size

    500ug

  • Price

    $4,635

1

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