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WZA4139-100ul
100uL
$795
| target | NEFL |
| host | Mouse |
| gene id | 4747 |
| reactivity | Human, Mouse |
| clonality | Monoclonal |
| clone number | K49012_6C10 |
| iso type | IgG1 |
| conjugate | Unconjugated |
| applications | IHC, ICC, WB, ELISA |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | IHC 1:200, WB 1:1000, ELISA 1:250-1:500 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
ELISA: Microtiter wells were coated with K49012_6C10 at 3 ug/mL as the capture antibody. NEFL (Cat. PP-106) was used as the antigen. Peroxidase conjugated mouse anti-human NEFL monoclonal antibody (K49012_4D10) was used as the detection antibody. Result: K49012_6C10 and K49012_4D10 can be used as a matched antibody pair to detect and quantify the concentration of NEFL.
Immunohistochemistry: IHC-P analysis of brain tissue by anti-NEFL antibody (K49012_6C10). IHC-P was performed using sections of the formalin-fixed paraffin-embedded brain tissue.
Western Blot: 15 ug of SH-SY5Y lysate and 15 ug of mouse brain tissue lysate were run on 6-18% SDS- PAGE under reducing conditions and blotted onto nitrocellulose membrane. K49012_6C10 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. NEFL band was visualized using ECL Substrate. Lane 1: 15 ug of SH-SY5Y lysate Lane 2: 15 ug of mouse brain tissue lysate Result: K49012_6C10 can detect NEFL by Western blotting.
WZA4139-100ul
100uL
$795