Human NEFL / Neurofilament (Ser2-Asp543) protein, with C-10xHis tag, produced in E. coli

PRODUCT

This protein contains a His tag at the C-terminus. The protein has a calculated MW of 63.4 kDa. The protein migrates at 65-80 kDa in SDS-PAGE.

gene name	NEFL
gene id	UniProt P07196
host species	Human
production host	E. coli
tags	C-10xHis
purity	>95% as determined by SDS-PAGE.
endotoxin	No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).

formulation	PBS, pH7.4
reconstitution	Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping	Shipped at ambient conditions
storage	Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

ELISA: Immobilized Human NFL Protein at 1 ug/mL (100 uL/well) binds to NFL Monoclonal Antibody with a linear range of 0.06-4 ng/mL .

SDS-PAGE analysis of NEFL protein