Human CLDN18.2 Full Length Transmembrane Protein (VLP, Virus-like Particle)

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PRODUCT

  • SKU

    DMF1022-50ug

  • Size

    50 ug

  • Price

    $1,800

1
  • SKU

    DMF1022-100ug

  • Size

    100 ug

  • Price

    $2,400

1
Virus-like particles (VLPs) encapsulated by cell membrane harboring target full-length multi-transmembrane protein are produced by co-transfection of HEK293 cells with genes encoding the target membrane protein and a virus structure protein. When VLPs are self-assembled and released from host cells, they are encapsulated by cell membrane overexpressig the target protein. Membrane proteins displayed on the surface of VLPs are execellent immunogens in its natural configuration. This Human CLDN18.2 Protein has a predicted MW of 29 kDa..Due to PTM, This actual MW on SDS-PAGE gel is around 50kDa..
target CLDN18.2
gene name CLDN18
alias Claudin 18.2
gene id Uniprot ID: P56856
tags None
conjugate None
host species Human
production host HEK293
formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize with occasional gentle mixing. Do not use solvents with pH lower than 6.5 in subsequent experiments.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

ELISA plates were pre-coated with 0.5 ug per well purified human CLDN18.2 VLP. Serial diluted Anti-CLDN18.2 monoclonal antibody (Zolbetuximab biosililar; IMAB362) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for IMAB362 binding with CLDN18.2 VLP is 15.37 ng/ml.

FACS analysis of CLDN18.2 VLP. A. Negative Control 1: CLDN18.2 VLP were stained only with Goat anti-human IgG Fc-PE secondary antibody. B. Negative Control 2: Control VLP were stained with anti-CLDN18.2 antibody (Zolbetuximab biosimilar; IMAB362) at 1 ug/ml, followed by Goat anti-human IgG Fc-PE secondary antibody. C. Negative Control 3: CLDN18.2 VLP were stained with anti-BCMA antibody?an irrelevant antibody?at 1 ug/ml, followed by Goat anti-human IgG Fc-PE secondary antibody. D. CLDN18.2 VLP were stained with anti-CLDN18.2 antibody?Zolbetuximab biosimilar; IMAB362?at 1 ug/ml, followed by Goat anti-human IgG Fc-PE secondary antibody.


PRODUCT

  • SKU

    DMF1022-50ug

  • Size

    50 ug

  • Price

    $1,800

1
  • SKU

    DMF1022-100ug

  • Size

    100 ug

  • Price

    $2,400

1