Human CLDN18.2 Full Length Transmembrane Protein (Synthetic Nanodisc) with Flag Tag

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PRODUCT

  • SKU

    DMF1028-10ug

  • Size

    10 ug

  • Price

    $1,350

1
  • SKU

    DMF1028-50ug

  • Size

    50 ug

  • Price

    $6,600

1
  • SKU

    DMF1028-100ug

  • Size

    100 ug

  • Price

    $12,000

1
Synthetic nanodiscs are produced by solubilizing HEK293 cell membrane fractions overexpessing target full-length multi-transmembrane protein with synthetic polymers such as styrene-maleic acid co-polymers (SMAs), forming discoidal nanostructures with target protein intact in lipid bilayer. The nanodiscs are further purified, offering intact membrane proteins in their natural configuration. This human full length CLDN18.2 Protein has a MW of 27.5 kDa.
target CLDN18.2
gene name CLDN18
alias Claudin 18.2
gene id Uniprot ID: P56856
tags C-Flag Tag
conjugate None
host species Human
production host HEK293
formulation Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize with occasional gentle mixing. Do not use solvents with pH lower than 6.5 in subsequent experiments.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

Elisa plates were added with Flag Tag CLDN18.2-Nanodisc (0.2 ug per well ) on an anti-Flag monoclonal antibody pre-coated (0.2 ug per well ) plate. Serial diluted anti- CLDN18.2 monoclonal antibodysolutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti- CLDN18.2 monoclonal antibody binding with CLDN18.2-Nanodisc is 593.6 ng/ml.

Human CLDN18.2-Nanodisc, Flag Tag on SDS-PAGE

Human CLDN18.2-Nanodisc can bind Anti-CLDN18.2 antibodywith an affinity constant of 1.619 nM as determined in a SPR assay.


PRODUCT

  • SKU

    DMF1028-10ug

  • Size

    10 ug

  • Price

    $1,350

1
  • SKU

    DMF1028-50ug

  • Size

    50 ug

  • Price

    $6,600

1
  • SKU

    DMF1028-100ug

  • Size

    100 ug

  • Price

    $12,000

1