Human CLDN18.2 Full Length Transmembrane Protein Fused with EGFP (VLP, Virus-like Particle)

Home/ Products / Full-length Membrane Proteins

PRODUCT

  • SKU

    DMF1024-25test

  • Size

    25 test

  • Price

    $270

1
  • SKU

    DMF1024-100test

  • Size

    100 test

  • Price

    $795

1
Virus-like particles (VLPs) encapsulated by cell membrane harboring target full-length multi-transmembrane protein are produced by co-transfection of HEK293 cells with genes encoding the target membrane protein and a virus structure protein. When VLPs are self-assembled and released from host cells, they are encapsulated by cell membrane overexpressig the target protein. Membrane proteins displayed on the surface of VLPs are execellent immunogens in its natural configuration. This human full length CLDN18.2 fusion protein has a MW of 54.2 kDa.
target CLDN18.2
gene name CLDN18
alias Claudin 18.2
gene id Uniprot ID: P56856
tags None
conjugate EGFP
host species Human
production host HEK293
formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize with occasional gentle mixing. Do not use solvents with pH lower than 6.5 in subsequent experiments.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

ELISA plates were pre-coated with 0.5 ug per well purified human CLDN18.2-EGFP-VLP. Serial diluted Anti-CLDN18.2 monoclonal antibodysolutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for anti- CLDN18.2 monoclonal antibody binding with CLDN18.2-EGFP-VLP is 877.5 ng/ml.

FACS analysis of CLDN18.2 VLP A. Negative Control 1: Expi293 cells were stained with Fluorescent Human CLDN18.2 Full Length Protein-VLP (EGFP). B. Negative Control 2: Anti-GPRC5D-CAR-Expi293 cells (an irrelevant CAR) were stained with Fluorescent Human CLDN18.2 Full Length Protein-VLP (EGFP). C. Positive Control: Anti-CLDN18.2-CAR-Expi293 cells were stained with biotin labeled Protein L, followed by streptavidin-APC antibody. D. Anti-CLDN18.2-CAR-Expi293 cells were stained with Fluorescent Human CLDN18.2 Full Length Protein-VLP (EGFP).


PRODUCT

  • SKU

    DMF1024-25test

  • Size

    25 test

  • Price

    $270

1
  • SKU

    DMF1024-100test

  • Size

    100 test

  • Price

    $795

1