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WZA4300-100ul
100uL
$720
| target | CD81 |
| host | Mouse |
| gene id | 975 |
| reactivity | Human |
| clonality | Monoclonal |
| clone number | K70020_12C5 |
| iso type | IgG2b |
| conjugate | Unconjugated |
| applications | IHC, ICC, IP, ELISA |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | IHC 1:200, IP 1:100, ELISA 1:250-1:500 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
ELISA: Microtiter wells were coated with K70020_15E5 at 3.0 ug/mL as the capture antibody. CD81 (Cat. PQ-088) was used as the antigen. Peroxidase conjugated mouse anti-human CD81 monoclonal antibody (K70020_12C5) was used as the detection antibody. Result: K70020_12C5 and K70020_15E5 can be used as a matched antibody pair to detect and quantify the concentration of CD81 .
Immunohistochemistry: IHC-P analysis of tonsil tissue by CD81 antibody (K70020_12C5). IHC-P was performed using sections of the formalin-fixed paraffin-embedded tonsil tissue.
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug K70020_12C5 with SK-MEL- 28 lysate containing 200 ug total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. Anti- CD81 (K70020_22A5) at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: CD81 immunoprecipitated from SK-MEL-28 lysate by K70020_12C5 Lane 2: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K70020_12C5 can immunoprecipitate CD81.
WZA4300-100ul
100uL
$720