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WZA3899-100ul
100uL
$650
| target | TNF |
| host | Mouse |
| gene id | 7124 |
| reactivity | Human |
| clonality | Monoclonal |
| clone number | KT31 |
| iso type | IgG2a |
| conjugate | Unconjugated |
| applications | WB, ELISA |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | WB 1:1000, ELISA 1:250-1:500 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Cross reactivity test: Microtiter wells were coated with various human cytokines. KT31 was used as primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Result: KT31 does not cross-react with human IL1B, IL2, IL4, IL5, IL6, IL7, CXCL8, IL9, IL10, IL11, IL12, IL13, IL17A, CSF2, IFNA, IFNG, CCL2 and KITLG.
ELISA: Microtiter wells were coated with KT31 at 3 ug/mL as the capture antibody. Human TNF was used as the antigen. Peroxidase conjugated mouse anti-human TNF (K06288_1B2) was used as the detection antibody. Result: KT31 and K06288_1B2 can be used as a matched antibody pair to detect and quantify the concentration of human TNF.
Western Blot: 25 ng of recombinant human TNF was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. KT31 at 1 ug/mL was used as primary antibody and peroxidase conjugated goat anti-mouse IgG was used as secondary antibody. TNF band was visualized using ECL Substrate. Result: KT31 can detect recombinant human TNF by Western blotting.
WZA3899-100ul
100uL
$650