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WZA3968-100ul
100uL
$505
| target | TNF |
| gene id | 7124 |
| reactivity | Human |
| host | Rat |
| clonality | Monoclonal |
| clone number | K03008_3A9 |
| iso type | IgG2a |
| conjugate | Unconjugated |
| applications | WB, ELISA |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | WB 1:1000, ELISA 1:250-1:500 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
ELISA: Microtiter wells were coated with K06288_1B2 at 3.0 ug/mL as the capture antibody. TNF (Cat. PQ-342) was used as the antigen. Peroxidase conjugated mouse anti-human TNF monoclonal antibody (K03008_3A9) was used as the detection antibody. Result: K03008_3A9 and K06288_1B2 can be used as a matched antibody pair to detect and quantify the concentration of TNF .
Western Blot: 25 ng of recombinant human TNF was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K03008_3A9 at 1 ug/mL was used as primary antibody and peroxidase conjugated goat anti-rat IgG was used as secondary antibody. TNF-alpha band was visualized using ECL Substrate. Result: K03008_3A9 can detect recombinant human TNF-alpha by Western blotting.
WZA3968-100ul
100uL
$505