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WZA4197-100ul
100uL
$650
| target | TNF |
| host | Mouse |
| gene id | 7124 |
| reactivity | Human |
| clonality | Monoclonal |
| clone number | K29020_5G7 |
| iso type | IgG2a |
| conjugate | Unconjugated |
| applications | IP, WB |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | WB 1:1000, IP 1:100 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Immunoprecipitation: Immunoprecipitation was performed by incubation of 2.5 ug K29020_5G7 with PMA, LPS and BFA treated THP-1 lysate containing 200 ug total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. TNF (K29020_9E6) at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody. The isotype control antibody was KT82. Lane 1: TNF immunoprecipitated from PMA, LPS and BFA treated THP-1 lysate by K29020_5G7 Lane 2: The same as Lane 2 but KT82 was used as IgG isotype control antibody Result: K29020_5G7 can immunoprecipitate TNF.
Western Blot: 25 ng of recombinant human TNF was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K29020_5G7 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. TNF band was visualized using ECL Substrate. Result: K29020_5G7 can detect recombinant human TNF by Western blotting.
WZA4197-100ul
100uL
$650