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WZA1341-100ul
100uL
$795
| target | Tau |
| host | Mouse |
| reactivity | Human, Rat, Mouse |
| clonality | Monoclonal |
| clone number | KT233 |
| iso type | IgG2b |
| conjugate | Unconjugated |
| applications | IHC, ICC, WB, ELISA |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | IHC 1:200, WB 1:1000, ELISA 1:250-1:500 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Cross reactivity test: Microtiter wells were coated with various peptides and recombinant proteins. KT233 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Result: KT233 can detect Tau proteins regardless of phosphorylation.
ELISA: Microtiter wells were coated with K40031_11H5 at 3 ug/mL as the capture antibody. Recommbinant pTau181 Protein (Cat. P00003CA) was used as the antigen. Peroxidase conjugated pTau181 Antibody (KT233) was used as the detection antibody. Result: KT233 and K40031_11H5 can be used as a matched antibody pair to detect and quantify the concentration of MAPT .
Immunohistochemistry: IHC-P analysis of brain tissue by Tau antibody (KT233). IHC-P was performed using sections of the formalin-fixed paraffin-embedded brain tissue.
Western Blot: Various protein samples were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. KT233 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Tau band was visualized using ECL Western Blotting Substrate. Lane 1: 15 ug of brain tissue lysate Lane 2: 15 ug of rat brain tissue lysate Lane 3: 15 ug of mouse brain tissue2 lysate Result: KT233 can detect Tau by Western blotting.
WZA1341-100ul
100uL
$795