Mouse anti-Human IgA Monoclonal Antibody (Clone KT41)

Cross reactivity test: Microtiter wells were coated with human immunoglobulins with various isotypes. KT41 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Result: KT41 does not cross-react with human IgG1, IgG2, IgG3, IgG4, IgM and IgE.

ELISA: Microtiter wells were coated with KT41 at 5.0 ug/mL as the capture antibody. IGHA1/IGHA2 (Cat. KC-006) was used as the antigen. Peroxidase conjugated mouse anti-human IGHA1/IGHA2 monoclonal antibody (KT40) was used as the detection antibody. Result: KT41 and KT40 can be used as a matched antibody pair to detect and quantify the concentration of IGHA1/IGHA2 .

Immunohistochemistry: IHC-P analysis of human tonsil tissue by anti-human IgA antibody (KT41). IHC-P was performed using sections of the formalin-fixed paraffin-embedded human tonsil tissue.

Western Blot: Human IgA, human IgM, human IgG and human IgE were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. KT41 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Human IgA band was visualized using ECL Western Blotting Substrate. Lane 1: 100 ng of human IgA Lane 2: 100 ng of human IgM Lane 3: 100 ng of human IgG Lane 4: 100 ng of human IgE Result: KT41 can specifically detect human IgA by Western blotting.