Mouse anti-Human IgG4 Monoclonal Antibody (Clone KT130)

Cross reactivity test: Microtiter wells were coated with human immunoglobulins with various isotypes. KT130 was used as primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Result: KT130 does not cross-react with human IgG1, IgG2, IgG3, IgA, IgM and IgE.

ELISA: Microtiter wells were coated with KT130 at 5.0 ug/mL as the capture antibody. IGHG4 (Cat. KC-004) was used as the antigen. Peroxidase conjugated mouse anti-human IGHG4 monoclonal antibody (KAA199_6A2) was used as the detection antibody. Result: KT130 and KAA199_6A2 can be used as a matched antibody pair to detect and quantify the concentration of IGHG4 .

Immunohistochemistry: IHC-P analysis of human tonsil tissue by anti-human IgG4 antibody (KT130). IHC-P was performed using sections of the formalin-fixed paraffin-embedded human tonsil tissue.

Western Blot: Various protein samples were run on 6-18% SDS-PAGE under reducing conditions and blotted onto PVDF membrane. KT130 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Human IgG4 band was visualized using ECL Western Blotting Substrate. Lane 1: 100 ng of human IgG1 Lane 2: 100 ng of human IgG2 Lane 3: 100 ng of human IgG3 Lane 4: 100 ng of human IgG4 Lane 5: 100 ng of human IgA Lane 6: 100 ng of human IgM Lane 7: 100 ng of human IgE Result: KT130 can specifically detect human IgG4 by Western blotting.