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WZA1174-100ul
100uL
$650
| target | Human IgG4 |
| gene id | 3503 |
| reactivity | Human |
| host | Mouse |
| clonality | Monoclonal |
| clone number | K06216_14H8 |
| iso type | IgG1 |
| conjugate | Unconjugated |
| applications | IHC, ICC, WB |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | IHC 1:1000, WB 1:1000 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Cross reactivity test: Microtiter wells were coated with human immunoglobulins with various isotypes. K06216_14H8 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Result: K06216_14H8 does not cross-react with human IgG1, IgG2, IgG3, IgA, IgM and IgE.
Immunohistochemistry: IHC-P analysis of human tonsil tissue by anti-human IgG4 antibody. Immunohistochemistry was performed using sections of formalin-fixed paraffin-embedded human tonsil tissue.
Western Blot: Various samples were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. K06216_14H8 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Human IgG4 band was visualized using ECL Western Blotting Substrate. Lane 1: 100 ng of human IgG1 Lane 2: 100 ng of human IgG2 Lane 3: 100 ng of human IgG3 Lane 4: 100 ng of human IgG4 Lane 5: 100 ng of human IgA Lane 6: 100 ng of human IgM Lane 7: 100 ng of human IgE Lane 8: 100 ng of rat IgG1 Result: K06216_14H8 can specifically detect human IgG4 by Western blotting.
WZA1174-100ul
100uL
$650