Mouse anti-Human IgG3 Monoclonal Antibody (Clone K16244_8D9)

Cross reactivity test: Microtiter wells were coated with human immunoglobulins with various isotypes. K16244_8D9 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Result: K16244_8D9 does not cross-react with human IgG1, IgG2, IgG4, IgA, IgM and IgE.

ELISA: Microtiter wells were coated with K16244_8D9 at 3 ug/mL as the capture antibody. Recombinant human IgG3 (Cat. KC-003) was used as the antigen. Peroxidase conjugated mouse anti-human IgG3 monoclonal antibody (K06089_8E4) was used as the detection antibody. Result: K16244_8D9 and K06089_8E4 can be used as a matched antibody pair to detect and quantify the concentration of human IgG3.

Immunohistochemistry: IHC-P analysis of human tonsil tissue by anti-human IgG3 antibody (K16244_8D9). IHC-P was performed using sections of the formalin-fixed paraffin-embedded human tonsil tissue.

Western Blot: Human IgG1, human IgG2, human IgG3, human IgG4, human IgA, human IgM, human IgE and rat IgG1 were run on 6-18% SDS-PAGE under reducing conditions and blotted onto PVDF membrane. K16244_8D9 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Human IgG3 band was visualized using ECL Western Blotting Substrate. Lane 1: 100 ng of human IgG1 Lane 2: 100 ng of human IgG2 Lane 3: 100 ng of human IgG3 Lane 4: 100 ng of human IgG4 Lane 5: 100 ng of human IgA Lane 6: 100 ng of human IgM Lane 7: 100 ng of human IgE Lane 8: 100 ng of rat IgG1 Result: K16244_8D9 can specifically detect human IgG3 by Western blotting.