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WZA1167-100ul
100uL
$650
| target | Human IgA |
| gene id | 3494 |
| reactivity | Human |
| host | Mouse |
| clonality | Monoclonal |
| clone number | KT153 |
| iso type | IgG2b |
| conjugate | Unconjugated |
| applications | IHC, ICC, WB |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | IHC 1:500, WB 1:1000 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Cross reactivity test: Microtiter wells were coated with human immunoglobulins with various isotypes. KT153 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Result: KT153 reacts with human IgA1 and IgA2. KT153 does not cross-react with human IgG1, IgG2, IgG3, IgG4, IgM and IgE.
Immunohistochemistry: IHC-P analysis of tonsil tissue by anti-human IgA antibody (KT153). Immunohistochemistry was performed using sections of the formalin-fixed paraffin-embedded tonsil tissue.
Western Blot: Human IgM, human IgG, human IgE and human IgA were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. KT153 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Human IgA band was visualized using ECL Western Blotting Substrate. Lane 1: 100 ng of human IgM Lane 2: 100 ng of human IgG Lane 3: 100 ng of human IgE Lane 4: 100 ng of human IgA Result: KT153 can specifically detect human IgA by Western blotting.
WZA1167-100ul
100uL
$650