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WZA3987-100ul
100uL
$650
| target | Human IgG |
| reactivity | Human |
| host | Mouse |
| clonality | Monoclonal |
| clone number | KT131 |
| iso type | IgG2a |
| conjugate | Unconjugated |
| applications | IHC, ICC, WB |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | IHC 1:2000, WB 1:1000 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Cross reactivity test: Microtiter wells were coated with human immunoglobulins with various isotypes. KT131 was used as primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Result: KT131 does not cross-react with human IgA, IgM and IgE.
Immunohistochemistry: IHC-P analysis of human tonsil tissue by anti-human IgG antibody (KT131). IHC-P was performed using sections of the formalin-fixed paraffin-embedded human tonsil tissue.
Western Blot: Various protein samples were run on 6-18% SDS-PAGE under reducing conditions and blotted onto PVDF membrane. KT131 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Human IgG band was visualized using ECL Western Blotting Substrate. Lane 1: 100 ng of human IgG1 Lane 2: 100 ng of human IgG2 Lane 3: 100 ng of human IgG3 Lane 4: 100 ng of human IgG4 Lane 5: 100 ng of human IgA Lane 6: 100 ng of human IgM Lane 7: 100 ng of human IgE Result: KT131 can specifically detect human IgG by Western blotting.
WZA3987-100ul
100uL
$650