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WZA1189-100ul
100uL
$650
| target | Rat IgG1 |
| reactivity | Rat |
| host | Mouse |
| clonality | Monoclonal |
| clone number | KT96 |
| iso type | IgG1 |
| conjugate | Unconjugated |
| applications | WB, ELISA |
| formulation | Supplied as solution in phosphate buffered saline containing 0.09% sodium azide |
| dilution | WB 1:1000, ELISA 1:250-1:500 |
| shipping | Ship at ambient conditions or with ice packs. |
| storage | Store at 4°C. The antibody is stable for at least one month from date of shipment. For long-term storage, aliquote the antibody and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
ELISA: Microtiter wells were coated with KT96 at 3 ug/mL as the capture antibody. Recommbinant Rat IgG1 Protein (Cat. KC-188) was used as the antigen. Peroxidase conjugated Anti-Rat IgG1 Antibody (KT149) was used as the detection antibody. Result: KT96 and KT149 can be used as a matched antibody pair to detect and quantify the concentration of Rat IgG1 .
Western Blot: Various protein samples were run on 6-18% SDS-PAGE under reducing conditions and blotted onto PVDF membrane. KT96 at 1 ug/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Rat IgG1 band was visualized using ECL Western Blotting Substrate. Lane 1: 100 ng of rat IgG1 Lane 2: 100 ng of rat IgG2a Lane 3: 100 ng of rat IgG2b Lane 4: 100 ng of rat IgG2c Lane 5: 100 ng of rat IgM Result: KT96 can specifically detect rat IgG1 by Western blotting.
WZA1189-100ul
100uL
$650